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Single-cell controlled human induced pluripotent stem cell differentiation to pericytes by cmos[...]

Louvain
Imec India Private Limited
De 100 000 € à 125 000 € par an
Publiée le Publiée il y a 4 h
Description de l'offre

Overview Single‑cell controlled human induced pluripotent stem cell differentiation to pericytes by CMOS‑MEA‑assisted electroporation. The role will involve using the CMOS‑MEA platform to deliver genetic molecules at very low voltages (~1 V) to direct hiPSC differentiation toward brain‑specific pericytes, study expression of key markers such as PDGFRβ, CD13, CD146, NKX3.1, CXCL12, and the Notch3 intracellular domain (N3ICD), and develop microscale co‑culture systems with endothelial cells.

Key Responsibilities

Apply precise electroporation on single‑cell resolution using CMOS‑MEA chips to introduce differentiation factors into hiPSCs.

Monitor differentiation and confirm marker expression (PDGFRβ, CD13, CD146, NKX3.1, CXCL12, N3ICD).

Pattern differentiated cells at subcellular resolution and create micropatterned 2D vasculature‑like EC and PC co‑cultures.

Collaborate with the endothelial‑cell differentiation team to carry out co‑transfections and optimise co‑culture conditions.

Record experimental data, analyse results, and contribute to the scientific analysis and reporting of findings.

Qualifications Type of internship : Master internship Duration : 2‑6 months Required educational background : Biomedical engineering, Physics, or related fields University promotor : Liesbet Lagae (KU Leuven)

Application For further information or for application, please contact Hanna Kuzminska (Hanna.Kuzminska@imec.be) and Yoke Chin Chai (Yoke.Chin.Chai@imec.be). The reference code for this position is

2026-INT-107. Mention this reference code in your application. Only for self‑supporting students. Incomplete applications will not be considered.

Applications should include the following information: resume motivation current study

imec's cleanroom #J-18808-Ljbffr

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